Establishment and characterization of a cell line from a malignant fibrous histiocytoma of bone developing in a patient with multiple fibrous dysplasia.
J Cancer Res Clin Oncol
; 128(1): 45-9, 2002 Jan.
Article
em En
| MEDLINE
| ID: mdl-11862471
ABSTRACT
PURPOSE AND METHODS:
In order to provide material for genetic analysis of fibrous dysplasia (FD), a cell line designated GBS-1 was established from a secondary bone malignant fibrous histiocytoma (MFH) developing in a rib of a 44-year-old male polyostotic FD patient.RESULTS:
The GBS-1 cells are characterized by a pleomorphic spindle cell morphology with abundant mucus production. On transplantation to nude mouse subcutis the cell line forms myxoid-spindle cell sarcomas with giant cells, the myxoid product being positive for periodic acid-Schiff (PAS) and alcian blue (Al-B) stains and completely digested by hyaluronidase, mimicking the original tumor. Chromosome and genetic analyses revealed multiple structural and numerical abnormalities of chromosomes with a large number of unidentifiable chromosomes and p53 mutation in exon 7 with LOH in the counterpart.CONCLUSIONS:
Since cell lines for FD have hitherto not been available, the GBS-1 cells should prove useful for genetic analyses of FD and also MFH of bone origin.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias Ósseas
/
Histiocitoma Fibroso Benigno
/
Técnicas de Cultura de Células
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Displasia Fibrosa Óssea
Limite:
Adult
/
Animals
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Humans
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Male
Idioma:
En
Revista:
J Cancer Res Clin Oncol
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Japão