Recruitment of scribble to the synaptic scaffolding complex requires GUK-holder, a novel DLG binding protein.
Curr Biol
; 12(7): 531-9, 2002 Apr 02.
Article
em En
| MEDLINE
| ID: mdl-11937021
ABSTRACT
BACKGROUND:
Membrane-associated guanylate kinases (MAGUKs), such as Discs-Large (DLG), play critical roles in synapse maturation by regulating the assembly of synaptic multiprotein complexes. Previous studies have revealed a genetic interaction between DLG and another PDZ scaffolding protein, SCRIBBLE (SCRIB), during the establishment of cell polarity in developing epithelia. A possible interaction between DLG and SCRIB at synaptic junctions has not yet been addressed. Likewise, the biochemical nature of this interaction remains elusive, raising questions regarding the mechanisms by which the actions of both proteins are coordinated.RESULTS:
Here we report the isolation of a new DLG-interacting protein, GUK-holder, that interacts with the GUK domain of DLG and which is dynamically expressed during synaptic bouton budding. We also show that at Drosophila synapses DLG colocalizes with SCRIB and that this colocalization is likely to be mediated by direct interactions between GUKH and the PDZ2 domain of SCRIB. We show that DLG, GUKH, and SCRIB form a tripartite complex at synapses, in which DLG and GUKH are required for the proper synaptic localization of SCRIB.CONCLUSIONS:
Our results provide a mechanism by which developmentally important PDZ-mediated complexes are associated at the synapse.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sinapses
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Núcleosídeo-Fosfato Quinase
/
Proteínas de Insetos
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Proteínas de Drosophila
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Proteínas Supressoras de Tumor
/
Proteínas de Membrana
Limite:
Animals
Idioma:
En
Revista:
Curr Biol
Assunto da revista:
BIOLOGIA
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Estados Unidos