S antigen specific effector T cell activation detected by cytokine flow cytometry.
Br J Ophthalmol
; 86(5): 517-20, 2002 May.
Article
em En
| MEDLINE
| ID: mdl-11973244
ABSTRACT
BACKGROUND/AIMS:
Effector T cell activation is particularly important in the initiation of autoimmune uveitis. This pilot study seeks to demonstrate activation of human peripheral effector T cells in response to the uveitis candidate autoantigen, retinal S antigen (SAg), using cytokine flow cytometry (CFC).METHODS:
Peripheral blood mononuclear cell (PBMC) suspensions from uveitis patients and controls were stimulated with bovine SAg. Activation responses were detected by CFC.RESULTS:
Electronic gating enabled analysis of CD69+, IFN-gamma+ CD4+ lymphocytes. An SAg specific response was detectable in four of 13 patients and four of eight controls.CONCLUSION:
SAg specific, peripheral, effector T cell activation can be detected by CFC. Similar levels of responsiveness were seen in patient and control groups. More detailed cytokine profiling may demonstrate functional differences between the groups.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Doenças Autoimunes
/
Uveíte
/
Ativação Linfocitária
/
Linfócitos T
/
Arrestina
Limite:
Humans
Idioma:
En
Revista:
Br J Ophthalmol
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Reino Unido