Differential roles of two SARP-encoding regulatory genes during tylosin biosynthesis.
Mol Microbiol
; 43(2): 449-58, 2002 Jan.
Article
em En
| MEDLINE
| ID: mdl-11985721
ABSTRACT
The tylosin biosynthetic gene cluster of Streptomyces fradiae is remarkable in harbouring at least five regulatory genes, two of which (tylS and tylT) encode proteins of the Streptomyces antibiotic regulatory protein (SARP) family. The aim of the present work was to assess the respective contributions of TylS and TylT to tylosin production. A combination of targeted gene disruption, fermentation studies and gene expression analysis via reverse transcriptase-polymerase chain reaction (RT-PCR) suggests that tylS is essential for tylosin production and controls the expression of tylR (previously shown to be a global activator of the biosynthetic pathway) plus at least one other gene involved in polyketide metabolism or regulation thereof. This is the first demonstration of a SARP acting to control another regulatory gene during antibiotic biosynthesis. In contrast, tylT is not essential for tylosin production.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Streptomyces
/
Proteínas de Bactérias
/
Genes Bacterianos
Idioma:
En
Revista:
Mol Microbiol
Assunto da revista:
BIOLOGIA MOLECULAR
/
MICROBIOLOGIA
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Reino Unido