In situ detection of cellular and abnormal isoforms of prion protein in brains of cattle with bovine spongiform encephalopathy and sheep with scrapie by use of a histoblot technique.
J Vet Diagn Invest
; 14(3): 255-7, 2002 May.
Article
em En
| MEDLINE
| ID: mdl-12033685
ABSTRACT
To detect prion protein, brains from 5 cattle naturally affected with bovine spongiform encephalopathy (BSE) and 3 sheep naturally affected with scrapie were examined and compared with brains of normal cattle and sheep using a histoblot technique. The technique enabled the in situ distinctive detection of the cellular (PrP(C)) and abnormal (PrP(Sc)) isoforms of the prion protein. In BSE- or scrapie-affected brains, the Prp(C) signal decreased, especially in those areas where the PrP(Sc) signal was detected.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Scrapie
/
Príons
/
Imuno-Histoquímica
/
Encefalopatia Espongiforme Bovina
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
Limite:
Animals
Idioma:
En
Revista:
J Vet Diagn Invest
Assunto da revista:
MEDICINA VETERINARIA
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Japão