Antigenic characterization of morphologically distinct Anaplasma marginale isolates using a panel of monoclonal antibodies.
Vet Parasitol
; 107(1-2): 169-77, 2002 Jul 29.
Article
em En
| MEDLINE
| ID: mdl-12072223
ABSTRACT
The present study, describes the antigenic characterization of a Brazilian isolate of Anaplasma marginale with appendage (tail). A panel of monoclonal antibodies (McAbs) was produced and tested by the indirect fluorescent antibody test (IFAT), ELISA and Western blotting, and used to characterize two isolates of A. marginale (one with appendage and another without appendage). Among the clones produced, eight recognized antigenic proteins, with molecular weights varying from 18.4 to 66kDa. In Western blotting, the McAb reacted against a 45kDa antigen, which was shown, by the IFAT, to be located in the tail. Immunocytochemistry confirmed the tail specificity of the monoclonal reacting against the 45kDa antigen. The panel of McAb produced has a potential use in discriminating morphologically distinct A. marginale isolates. The present study, demonstrates the occurrence of antigenic diversity among Brazilian isolates of A. marginale.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Variação Antigênica
/
Anaplasma
/
Antígenos de Bactérias
Limite:
Animals
País/Região como assunto:
America do sul
/
Brasil
Idioma:
En
Revista:
Vet Parasitol
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Brasil