Serial analysis of cytokine mRNA profiles in whole blood samples from patients with early multiple sclerosis.
J Neurol Sci
; 200(1-2): 53-5, 2002 Aug 15.
Article
em En
| MEDLINE
| ID: mdl-12127676
ABSTRACT
In this pilot study, we serially determined the cytokine messenger RNA (mRNA) expression pattern in whole blood samples from 12 patients with clinical isolated syndrome suggestive of early multiple sclerosis (MS) using a new sensitive quantitative polymerase chain reaction (PCR) method. Significantly higher levels of tumor necrosis factor-alpha (TNF-alpha; x 5.1), interferon-gamma (IFN-gamma; x 4.8) and interleukin-10 (IL-10; x 5.6) mRNA were detected in MS patients at the time of a relapse compared to healthy controls. Treatment with i.v. methylprednisolone (MP) led to an increase of IL-4 mRNA and a significant decrease of IFN-gamma and TNF-alpha mRNA expression. In this cohort of clinically stable patients, proinflammatory cytokines remained low during the 1-year follow-up period. As several indications point to a cytokine dysregulation in MS, quantitative analysis of cytokine mRNA profiles in whole blood samples by real time PCR may be a useful immunological marker to monitor disease activity in future therapeutic trials in MS.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
RNA Mensageiro
/
Citocinas
/
Esclerose Múltipla
Tipo de estudo:
Observational_studies
/
Prognostic_studies
Limite:
Adult
/
Female
/
Humans
/
Male
Idioma:
En
Revista:
J Neurol Sci
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Alemanha