Asaia sp., an unusual spoilage organism of fruit-flavored bottled water.
Appl Environ Microbiol
; 68(8): 4130-1, 2002 Aug.
Article
em En
| MEDLINE
| ID: mdl-12147519
ABSTRACT
A gram-negative bacillus was isolated from a batch of fruit-flavored bottled water, which had spoiled as a result of bacterial overgrowth (>10(6) CFU/ml). The spoilage organism was extremely difficult to identify phenotypically and was poorly identified as Pasturella sp. (78.7% identification profile) employing the API 20NE identification scheme, which gave the profile 5040000. Molecular identification through PCR amplification of a partial region of the 16S rRNA gene followed by direct automated sequencing of the PCR amplicon allowed identification of the organism. Due to the sequence identity (100%) between the spoilage organism and a reference strain in GenBank, the spoilage isolate was considered to be an Asaia sp., a recently described genus and member of the acetic acid bacteria. This is the first report of Asaia sp. causing spoilage of a foodstuff and highlights the benefits of molecular identification techniques based on 16S rRNA gene sequences in the identification of unusual spoilage organisms.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Microbiologia da Água
/
Bebidas
/
Acetobacteraceae
/
Frutas
Idioma:
En
Revista:
Appl Environ Microbiol
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Reino Unido