Specific fluorescent tracers. Imaging and applications for photodynamic therapy.
C R Biol
; 325(4): 487-93, 2002 Apr.
Article
em En
| MEDLINE
| ID: mdl-12161928
ABSTRACT
Our main objective is to enlarge the fluorescence use in biosciences, with especially the photodynamic therapy (PDT) used for cancer treatment as one of the target applications. Meta-tetra(hydroxyphenyl)chlorin (m-THPC) is a second-generation photosensitiser, applied in photodynamic therapy. The localisation of this sensitiser as well as its induced cell death mechanisms in human breast cancer cells (MCF-7 and its resistant subline MCF-7DXR, DXR doxorubicin) were evaluated using fluorescence microscopy. In addition, we will present two additional routes, whose aims are to create new features to respond to the PDT questioning firstly, the synthesis of fluorescent tracers, with a particular attention to the presence of hydrophilic groups (glucosamine ring) on the basic fluorophore structure to orientate the localisation of the probe and, secondly, the use of scanning near-field optical microscopy to reach a better resolution for the fluorescence microscopy analysis.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fotoquimioterapia
/
Corantes Fluorescentes
/
Microscopia de Fluorescência
Limite:
Female
/
Humans
Idioma:
En
Revista:
C R Biol
Assunto da revista:
BIOLOGIA
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
França