The detection of Mycobacterium leprae protein and carbohydrate antigens in skin and nerve from leprosy patients with type 1 (reversal) reactions.
Am J Trop Med Hyg
; 66(4): 409-15, 2002 Apr.
Article
em En
| MEDLINE
| ID: mdl-12164297
ABSTRACT
Type 1 (reversal) reactions are the most common immunological complications of leprosy. These episodes of delayed hypersensitivity produce severe local immunopathology and ultimately nerve damage. To date, the Mycobacterium leprae antigens associated with type 1 reactions have not been identified. Using monoclonal antibodies to defined protein and carbohydrate M. leprae epitopes (65, 35 and 28 kd and lipoarabinomannan [LAM]) in a two-step immunoperoxidase staining technique, M. leprae antigens were demonstrated in skin and nerve biopsies from patients in reversal reaction. Antigen presence and staining patterns were similar in skin and nerve lesions, implying that the pathological processes are similar in the two sites. Antigens were present both in macrophages and Schwann cells but also as a diffuse extracellular infiltrate associated with the inflammatory infiltrate. The 28-kd antigen was present most strongly and may be a potential candidate antigen for initiating type 1 reactions. LAM also stained strongly and persisted after treatment. The possible roles of LAM and 65 kd in the cellular events of type 1 reactions are discussed.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Nervos Periféricos
/
Pele
/
Proteínas de Bactérias
/
Hanseníase Dimorfa
/
Hipersensibilidade Tardia
/
Mycobacterium leprae
/
Antígenos de Bactérias
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Revista:
Am J Trop Med Hyg
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Reino Unido