Densitometric analysis of kawain in kava-kava root extracts.
Acta Pol Pharm
; 58(6): 463-8, 2001.
Article
em En
| MEDLINE
| ID: mdl-12197620
ABSTRACT
A thin layer chromatographic-densitometric method for the identification and determination of kawain in a mixture of kavalactones obtained from a methanol extract of kava-kava roots (Piper methysticum) was developed. The main compounds present in the extract were separated with good results using silica gel F 254 coated TLC plates as the stationary phase and hexane-dioxane-butyl acetate-ammonia 25% (7230.5 v/v/v/v) as the mobile phase. The qualitative identification and quantitative measurement of kawain were obtained by UV measurements at lambda=250 nm. The described method demonstrates very high sensitivity with the limit of detection established at 20 ng. 99.38% of the kawain was recovered and the concentration range showed a linearity from 0.0005% to 0.004%. Under these conditions, the percent of kawain in the presence of other kavalactones in the prepared extracts (I-VII), was determined. Similar results were obtained for all extracts, with the kawain content ranging from 1.01% to 1.16%.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pironas
/
Kava
Tipo de estudo:
Qualitative_research
Idioma:
En
Revista:
Acta Pol Pharm
Ano de publicação:
2001
Tipo de documento:
Article
País de afiliação:
Polônia