[A thermostable Clostridium thermocellum lichenase-based reporter system for studying the gene expression regulation in prokaryotic and eukaryotic cells]. / Reporternaia sistema, osnovannaia na termostabil'nosti likhenazy Clostridium thermocellum, dlia izucheniia reguliatsii ékspressii genov v kletkakh pro- i éukarioticheskikh organizmov.
Mol Biol (Mosk)
; 36(5): 868-76, 2002.
Article
em Ru
| MEDLINE
| ID: mdl-12391851
ABSTRACT
A new reporter system was developed to study the gene expression regulation in prokaryotic (Escherichia coli) and eukaryotic (Saccharomyces cerevisiae and mammalian) cells. The system was based on the modified bacterial lichenase gene (licBM2), which was shown to meet the requirements for a reporter. The gene product was active and did not undergo modification in heterologous hosts. Simple and sensitive methods were used to detect and to quantitate the lichenase activity. Inducible licBM2 expression was demonstrated with E. coli and yeast cells, allowing the system to be employed in dynamic studies.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Engenharia Genética
/
Clostridium
/
Genes Reporter
/
Células Eucarióticas
/
Glicosídeo Hidrolases
Limite:
Animals
Idioma:
Ru
Revista:
Mol Biol (Mosk)
Ano de publicação:
2002
Tipo de documento:
Article