Purification and characterization of soluble peroxidase from oil palm (Elaeis guineensis Jacq) leaf.
Phytochemistry
; 61(5): 503-11, 2002 Nov.
Article
em En
| MEDLINE
| ID: mdl-12409016
ABSTRACT
Soluble peroxidase (POD) from oil palm leaf was purified by (NH(4))(2)SO(4) precipitation, anion exchange chromatography and molecular exclusion chromatography. The purification grade obtained was 429 yielding 54% of the enzyme activity. Electrophoresis of purified enzyme under denatured conditions revealed M(r) of 48+/-2 kDa. It has an optimum pH of 5 and it exhibited very high pH and thermal stabilities. K(m) for guaiacol, ABTS and pyrogallol were 3.96, 1 and 0.84 mM, respectively. Immunocytochemical localization studies showed that soluble POD was mainly located in the vascular bundles and epidermis of leaf.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peroxidases
/
Arecaceae
Idioma:
En
Revista:
Phytochemistry
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Índia