Molecular analysis of the complete set of length mutations found in the plastomes of Triticum-Aegilops species.
Genome
; 45(5): 956-62, 2002 Oct.
Article
em En
| MEDLINE
| ID: mdl-12416629
ABSTRACT
Precise location and nature of each of 14 length mutations detected among chloroplast DNAs of Triticum-Aegilops species by RFLP analysis were determined at the nucleotide sequence level. Each mutation was compared with at least three non-mutated wild-type plastomes as standards. These 14 length mutations were classified into 4 duplications and 10 deletions. One duplication occurred in the small single-copy region close to the border of the inverted repeat, and the remaining 13 length mutations took place in the large single-copy region. All length mutations occurred in the intergenic regions, suggesting that these length mutations do not affect plastid gene expression. Saltatory replication was the cause of all duplications, whereas intramolecular recombination mediated by short direct repeats played a substantial role in the deletions. Recurrent occurrences of certain deletion events were found in some AT-rich regions, which constituted hot spots for deletion. Out of four hypervariable regions detected among the grass plastomes, two (downstream of rbcL and a tRNA gene accumulated region) were still active after differentiation of Triticum and Aegilops complex.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Triticum
/
DNA de Cloroplastos
/
Poaceae
/
Mutação
Idioma:
En
Revista:
Genome
Assunto da revista:
GENETICA
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Japão