Quantitative determination of bile salt hydrolase activity in bacteria isolated from the small intestine of chickens.
Appl Environ Microbiol
; 68(12): 6425-8, 2002 Dec.
Article
em En
| MEDLINE
| ID: mdl-12450872
ABSTRACT
A quantitative assay based on high-performance liquid chromatography analysis of bile salts and bacterial protein determination was established for investigating bile salt hydrolase (BSH) activity in bacteria isolated from the small intestine of chickens. Bacteria were isolated using various media and were subsequently grouped according to cell morphology, fermentation profile, and 16S ribosomal DNA sequence. Representative isolates from each bacterial group were assayed for BSH activity. The isolates differed in BSH activity with respect to the state of growth and preculturing with and without taurochenodeoxycholate. The highest levels of BSH activity were found with Enterococcus faecium and Clostridium perfringens.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bactérias
/
Amidoidrolases
/
Intestino Delgado
Limite:
Animals
Idioma:
En
Revista:
Appl Environ Microbiol
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Dinamarca