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Deglyco-peplomycin metal complexes on DNA fibers: a role of the sugar moiety for the stability and the orientation of the complexes.
J Biol Inorg Chem ; 8(1-2): 135-40, 2003 Jan.
Article em En | MEDLINE | ID: mdl-12459908
Binding structures of metal complexes of deglyco-peplomycin (dPEP) on DNA were investigated by comparing dPEP complexes with those of bleomycin (BLM) using DNA fiber EPR spectroscopy. A low spin species of Fe(III)dPEP observed in the DNA pellet changed irreversibly to several high spin species after the fabrication of the DNA fibers. The g values of the high spin species were different from those of Fe(III)BLM. The high spin species could not be nitrosylated reductively to ON-Fe(II)dPEP, suggesting that some nitrogen atoms coordinated to the Fe(III) were displaced on the DNA fibers. On the other hand, O(2)-Co(II)dPEP remained intact on the fibers similarly to O(2)-Co(II)BLM but with an increased randomness in the orientation on the DNA. In contrast to Cu(II)BLM, a considerable amount of Cu(II)dPEP bound almost randomly on B-form DNA fibers. These results indicated that the sugar moiety in peplomycin or bleomycin is playing an important role in enhancing the stability of the metal-binding domain and in the stereospecificity of the binding on DNA.
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: DNA / Compostos Ferrosos / Peplomicina / Adutos de DNA / Cobre / Antibióticos Antineoplásicos Limite: Animals Idioma: En Revista: J Biol Inorg Chem Assunto da revista: BIOQUIMICA Ano de publicação: 2003 Tipo de documento: Article País de publicação: Alemanha
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: DNA / Compostos Ferrosos / Peplomicina / Adutos de DNA / Cobre / Antibióticos Antineoplásicos Limite: Animals Idioma: En Revista: J Biol Inorg Chem Assunto da revista: BIOQUIMICA Ano de publicação: 2003 Tipo de documento: Article País de publicação: Alemanha