Relationship between the changes in cellular volume of fish spermatozoa and their cryoresistance.
Cryo Letters
; 23(6): 353-60, 2002.
Article
em En
| MEDLINE
| ID: mdl-12522505
ABSTRACT
We have investigated the hypothesis that the spermatozoa of marine fish are more resistant than freshwater species to the dynamic changes in osmotic pressure that occur during the process of cryopreservation. We show that while the spermatozoa of marine fish can be successfully activated across a wide range of osmotic pressures (0-2000 mOsmol/l), those of the freshwater species only survive activation within a more restricted range (0-300 mOsm/l). After freeze-thawing, up to 30 percent of motile cells were found in silver carp samples, while up to 90 percent of motile cells were observed in samples from the haarder (Mugil soiuy B). Haarder spermatozoa showed no change of cell volume after dilution in activating or cryoprotective media, while the silver carp spermatozoa responded by swelling and eventual cell disruption. We propose that the differences in cryoresistance of silver carp (Hypophthalmichthys molitrix V.) and haarder spermatozoa may be determined by the ability to preserve cellular volume under non-isotonic conditions.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Preservação do Sêmen
/
Criopreservação
/
Peixes
Limite:
Animals
Idioma:
En
Revista:
Cryo Letters
Assunto da revista:
BIOLOGIA
/
QUIMICA
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Ucrânia