Response surface optimization of the critical medium components for the production of alkaline protease by a newly isolated Bacillus sp.
J Pharm Pharm Sci
; 5(3): 272-8, 2002.
Article
em En
| MEDLINE
| ID: mdl-12553896
ABSTRACT
PURPOSE:
Optimization of the fermentation medium for maximum alkaline protease production was carried out using a new strain, Bacillus Sp. PE-11.METHODS:
The carbon source (glucose), the nitrogen source (peptone) and salt solution were selected to optimize. A 2(3 )full factorial composite experimental design and response surface methodology were used in the design of experiments and in the analysis of results. This procedure limited the number of actual experiments performed while allowing for possible interactions between the three components. RESULTS ANDDISCUSSION:
The optimum values for the tested variables for the maximum alkaline protease production were; glucose 7.798 (g/L), peptone 9.548 (g/L) and salt solution 8.757%. The maximum alkaline protease production was 4,98,123 PU/L. This method was efficient; only 20 experiments were necessary to assess these conditions, and model adequacy was very satisfactory, as the coefficient of determination was 0.941.CONCLUSIONS:
In the work, we have demonstrated the use of a central composite factorial design by determining the conditions leading to the high yield of enzyme production. Thus, smaller and less time consuming experimental designs could generally suffice for the optimization of many fermentation processes.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peptonas
/
Bacillus
/
Serina Endopeptidases
/
Glucose
Tipo de estudo:
Health_economic_evaluation
/
Prognostic_studies
Idioma:
En
Revista:
J Pharm Pharm Sci
Assunto da revista:
FARMACIA
/
FARMACOLOGIA
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Índia