Short-term androgen deprivation does not alter CaR and VDR mRNA expression in duodenal mucosa in male rats.
Indian J Exp Biol
; 40(7): 780-4, 2002 Jul.
Article
em En
| MEDLINE
| ID: mdl-12597546
ABSTRACT
Androgen deprivation is associated with decline in intestinal calcium absorption. The effect of androgen on CaR and VDR intestinal mucosa has not yet been studied. Calcium homeostasis, a real bone mineral density (aBMD, dual energy X-ray absorptiometry) and expression of CaR and VDR mRNA in duodenal mucosa of orchidectomized (ORX) and sham operated (Sham) adult Sprague Dawley rats at 4 week have been studied. There was no significant difference in serum calcium, alkaline phosphatase, calcidiol and calcitriol levels between both the groups. Serum testosterone (T) (ng/dl) and inorganic phosphorous (iP) (mg/dl) levels were significantly lower in ORX rats. As compared to sham rats, ORX rats had significant decline in in-vitro aBMD at proximal, middle and distal tibia, proximal, mid and distal femur and femoral neck (P < 0.05). Northern blot analysis revealed no significant alteration in the CaR and VDR mRNA expression in duodenal mucosa in ORX rats. CaR and VDR mRNA expression in duodenal mucosa is therefore, not affected by physiological concentrations of testosterone in rats.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Ligação ao Cálcio
/
RNA Mensageiro
/
Receptores de Calcitriol
/
Duodeno
/
Androgênios
Limite:
Animals
Idioma:
En
Revista:
Indian J Exp Biol
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Índia