Binding of antibromelain monomeric Fab' improves the stability of stem bromelain against inactivation.
Biochim Biophys Acta
; 1646(1-2): 131-5, 2003 Mar 21.
Article
em En
| MEDLINE
| ID: mdl-12637019
ABSTRACT
Antienzyme polyclonal antibodies against stem bromelain were raised in male albino rabbits and the Fab' monomers isolated from the IgG of the immune sera. Incubation of bromelain with the Fab' resulted in binding and gel filtration of the resulting complex suggested a 11 stoichiometry. Complexing with the Fab' resulted in significant stabilization of bromelain against thermal inactivation and alkaline pH.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fragmentos Fab das Imunoglobulinas
/
Bromelaínas
Limite:
Animals
Idioma:
En
Revista:
Biochim Biophys Acta
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
Índia