Application of an in vitro endopeptidase assay for detection of residual toxin activity in tetanus toxoids.
Dev Biol (Basel)
; 111: 327-32, 2002.
Article
em En
| MEDLINE
| ID: mdl-12678257
ABSTRACT
Tetanus vaccine is composed of chemically denatured tetanus toxin (TeNT), thus safety testing requires confirmation of freedom from residual and reversible toxicity. Currently, TeNT activity is estimated using in vivo assay models. Information that TeNT acts by selectively inactivating protein leading to the blocking of release of neurotransmitters has provided the opportunity to develop in vitro biochemical assay for toxin activity. In this study we describe development and use of an in vitro endopeptidase assay for detection of TeNT activity in toxoid vaccine formulations.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Endopeptidases
/
Toxina Tetânica
/
Toxoide Tetânico
/
Testes de Toxicidade
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Dev Biol (Basel)
Assunto da revista:
ALERGIA E IMUNOLOGIA
Ano de publicação:
2002
Tipo de documento:
Article
País de afiliação:
Reino Unido