[Bifunctional reporter genes: construction and expression in prokaryotic and eukaryotic cells]. / Bifunktsional'nye reporternye geny: konstruirovanie i ékspressiia v kletkakh pro- i éukariot.
Mol Biol (Mosk)
; 37(2): 356-64, 2003.
Article
em Ru
| MEDLINE
| ID: mdl-12723481
ABSTRACT
Bifunctional reporter proteins were constructed to combine Clostridium thermocellum lichenase (LicBM2) with Aequorea victoria green fluorescent protein (GFP) or with Escherichia coli beta-glucuronidase (GUS). The major properties of the initial proteins were preserved in the hybrid ones LicBM2 was active at 65 degrees C, GFP fluoresced, and GUS hydrolyzed its substrates. LicBM2 remained active after extension of its C of N end. Bifunctional reporter systems were shown to provide a convenient tool for studying the gene expression regulation in prokaryotic (E. coli) and eukaryotic (Saccharomyces cerevisiae, mammalian) cells, advantages of one reporter compensating for drawbacks of the other.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Células Procarióticas
/
Proteínas Recombinantes
/
Engenharia de Proteínas
/
Genes Reporter
/
Células Eucarióticas
Limite:
Animals
Idioma:
Ru
Revista:
Mol Biol (Mosk)
Ano de publicação:
2003
Tipo de documento:
Article