Development of a novel bacterial artificial chromosome cloning system for functional studies.
Plasmid
; 49(2): 184-7, 2003 Mar.
Article
em En
| MEDLINE
| ID: mdl-12726772
ABSTRACT
Bacterial artificial chromosome (BAC) cloning systems currently in use generate high quality genomic libraries for gene mapping, identification, and sequencing. However, the most commonly used BAC cloning systems do not facilitate functional studies in eukaryotic cells. To overcome this limitation, we have developed pEBAC190G, a new BAC vector that combines the features of the first generation PAC/BAC vectors with eukaryotic elements that facilitate the transfection, episomal maintenance, and functional analysis of large genomic fragments in eukaryotic cells. A number of different cloning strategies may be used to retrofit genomic fragments from existing libraries into the new vector. The system was tested by the retrofitting of a 170kb NotI genomic fragment from the RPCI-11 BAC library into the NotI site of pEBAC190G. Clones from any eukaryotic genomic library harboured in this vector can be transferred from bacteria directly to eukaryotic cells for functional analysis.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Clonagem Molecular
/
Cromossomos Artificiais Bacterianos
/
Vetores Genéticos
Idioma:
En
Revista:
Plasmid
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
Austrália