[The effect of the period of cryopreservative storage on the cryosurvival of human spermatozoa].
Zhonghua Nan Ke Xue
; 9(2): 122-3, 2003 Apr.
Article
em Zh
| MEDLINE
| ID: mdl-12749133
ABSTRACT
OBJECTIVES:
To study the effect of cryopreservative period on the cryosurvival of human spermatozoa and find out the optimal recovery time of cryopreservation.METHODS:
Eighty-eight semen samples were collected from normal donors and divided randomly into 5 groups according to the period of cryopreservative storage (1 d, 7 d, 30 d, 180 d, 300 d) in liquid nitrogen after being frozen by the programized, three-step freezing method. Fresh and frozen-thawed semen were examined by the routine analysis of semen and then the sperm recovery rate were calculated.RESULTS:
There were no significant differences in sperm recovery rate between group I and the others (P > 0.05). The period of cryopreservative storage in liquid nitrogen had no correlation with the cryosurvival of human spermatozoa (r = 0.05, P > 0.05).CONCLUSIONS:
It was indicated that freezing-thawing after 24 h would be helpful to the screening of semen donors in batches for donor insemination of human sperm bank.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Preservação do Sêmen
/
Espermatozoides
/
Criopreservação
Limite:
Humans
/
Male
Idioma:
Zh
Revista:
Zhonghua Nan Ke Xue
Assunto da revista:
MEDICINA REPRODUTIVA
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
China