Analysis of optically active compounds using conventional chromatography with a circular dichroism detector.
J Pharm Biomed Anal
; 32(4-5): 905-12, 2003 Aug 08.
Article
em En
| MEDLINE
| ID: mdl-12899976
ABSTRACT
Analysis of optically active compounds in complex samples is often based on chiral chromatography or capillary electrophoresis in order to separate the enantiomers. This requires a chiral reagent, when using conventional chromatography, or an expensive chiral column, or a chiral selector, when using capillary electrophoresis. The type of column, reagent, or additive depends highly on the compound to be analysed. A simple and generally applicable method is using a conventional HPLC column coupled to a CD detector. Separation of enantiomers is not required, as they can be identified by a positive or negative peak. A racemate does not produce a peak; neither does an optically inactive compound. The application of HPLC-CD for the identification of pharmacologically active compounds, such as dexamphetamine, 5-hydroxytryptophan, (-)-huperzine A, and interferon, as standards, in registered drugs, in falsifications, and in food supplements is described.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Preparações Farmacêuticas
/
Dicroísmo Circular
Idioma:
En
Revista:
J Pharm Biomed Anal
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
Holanda