A soluble recombinant fusion protein of the transmembrane envelope protein of equine infectious anaemia virus for ELISA.
Vet Microbiol
; 31(2-3): 127-37, 1992 Jun 01.
Article
em En
| MEDLINE
| ID: mdl-1320787
ABSTRACT
The use of the bacterial expression vector, pGex, to produce an abundant, soluble fusion protein of gp45 from equine infectious anaemia virus is described. Purification of the recombinant protein was achieved by one step affinity chromatography on immobilized glutathione using competitive elution so no harsh conditions were required. This provides a readily available antigen that is defined, plentiful and cheap. Yields of 3.5 mg of purified soluble protein/litre of bacterial culture were obtained. This antigen was found to be suitable for ELISA. Background reactivity to either the glutathione-S-transferase (GST) fusion partner by immune sera or the EIA-GST fusion protein by normal sera were negligible.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Recombinantes de Fusão
/
Proteínas do Envelope Viral
/
Vírus da Anemia Infecciosa Equina
/
Antígenos Virais
Limite:
Animals
Idioma:
En
Revista:
Vet Microbiol
Ano de publicação:
1992
Tipo de documento:
Article
País de afiliação:
Austrália