Gamma-irradiation potentiates L-arginine-dependent nitric oxide formation in mice.
Biochem Biophys Res Commun
; 186(3): 1423-8, 1992 Aug 14.
Article
em En
| MEDLINE
| ID: mdl-1324666
ABSTRACT
Gamma-irradiation of mongrel mice at a sublethal dose (700 Roentgen) enhanced the formation of nitric oxide (NO) in the liver, intestine, lung, kidney, brain, spleen or heart of the animals. NO formation was determined by the increase in intensity of the EPR signal due to trapping of NO into mononitrosyl iron complexes (MNIC) with exogenous diethyldithiocarbamate (DETC) injected intraperitoneally. The EPR signal of these MNIC-DETC complexes was characterized by g-factor values at g perpendicular values at g perpendicular = 2.035 and g parallel = 2.02 and a triplet hyperfine structure at g perpendicular. The NO synthase inhibitor, NG-nitro-L-arginine, prevented MNIC-DETC complex formation both in liver and intestine, demonstrating the involvement of endogenous NO formed. Thus, gamma-irradiation may enhance endogenous NO biosynthesis in these tissues, presumably by facilitating the entry of Ca2+ ions into the membrane as well as the cytosol of NO-producing cells through irradiation-induced membrane lesions.
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01-internacional
Base de dados:
MEDLINE
Assunto principal:
Arginina
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Baço
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Encéfalo
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Coração
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Rim
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Fígado
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Pulmão
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Óxido Nítrico
Limite:
Animals
Idioma:
En
Revista:
Biochem Biophys Res Commun
Ano de publicação:
1992
Tipo de documento:
Article