gamma-Interferon increases metaiodobenzylguanidine incorporation and retention in human neuroblastoma cells.
Cancer Res
; 52(18): 4960-4, 1992 Sep 15.
Article
em En
| MEDLINE
| ID: mdl-1325288
ABSTRACT
Iodine-labeled m-iodobenzylguanidine (MIBG) is a widely used radiopharmaceutical for both diagnosis and biologically targeted radiotherapy of neuroblastoma. However, resistance to the radiotherapeutic effects of MIBG is often encountered, mainly due to lack of MIBG accumulation by neoplastic cells. We have investigated whether the induction of neuroblastoma cell differentiation modifies MIBG incorporation and retention. LAN-5 cells were selected, due to their moderate ability to take up MIBG. Treatment of these cells with gamma-interferon (IFN-gamma) resulted in morphological changes accompanied by a significant increase in overall cell-associated MIBG. Desimipramine, but not reserpine, easily depleted IFN-gamma-treated LAN-5 cells of their MIBG content. This suggests that the mechanism involved is an uptake enhancement rather than an improved storage ability. Indeed, IFN-gamma induces de nov synthesis of MIBG receptor-transporters, as demonstrated by polymerase chain reaction amplification and semiquantitative analysis. Our results suggest that pretreating neuroblastoma patients with IFN-gamma before MIBG administration may enhance the efficacy of both biologically targeted radioimaging and therapy of this tumor.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Interferon gama
/
Iodobenzenos
Limite:
Humans
Idioma:
En
Revista:
Cancer Res
Ano de publicação:
1992
Tipo de documento:
Article
País de afiliação:
Itália