Differential effect in vitro of tumor necrosis factor-alpha (TNF) on normal and virus-infected erythroid progenitors from Friend virus (FVA)-infected mice.
Exp Hematol
; 20(11): 1271-7, 1992 Dec.
Article
em En
| MEDLINE
| ID: mdl-1337323
In vivo administration of tumor necrosis factor-alpha (TNF) suppresses both normal and Friend virus (FVA)-infected erythroid progenitor cells (CFU-E). To examine the mechanism of erythroid suppression by TNF, we examined TNF's direct effect on normal and virus-infected cells in vitro. Productively infected fibroblast cell lines, fresh acute virus-infected spleen cells, and virus-infected CFU-E were sensitive, whereas uninfected CFU-E were resistant to TNF cytotoxicity in vitro. When FVA-infected erythroblasts were depleted from the spleen cell population in vitro with antivirus antibodies, TNF suppression of the remaining (uninfected) cells was abrogated. In contrast, both normal and virus-infected macrophage progenitor cells and immature erythroid progenitor cells were equally sensitive to TNF cytotoxicity in vitro. Normal erythroblasts had significantly fewer TNF receptors than FVA-infected erythroblasts, which also were morphologically less mature. These results suggest that TNF can differentially suppress late-stage virus-infected erythroid progenitors in vitro.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Leucemia Eritroblástica Aguda
/
Células Precursoras Eritroides
/
Fator de Necrose Tumoral alfa
/
Vírus da Leucemia Murina de Friend
Limite:
Animals
Idioma:
En
Revista:
Exp Hematol
Ano de publicação:
1992
Tipo de documento:
Article
País de publicação:
Holanda