DNA polymorphisms in methicillin-susceptible and methicillin-resistant strains of Staphylococcus aureus.
J Clin Microbiol
; 30(8): 2092-6, 1992 Aug.
Article
em En
| MEDLINE
| ID: mdl-1354223
ABSTRACT
Restriction fragment length polymorphisms in methicillin-susceptible and methicillin-resistant (MRSA) strains of Staphylococcus aureus isolated in the same hospital over a 4-month period were studied by using SmaI and ApaI digestion of genomic DNA and pulsed-field gel electrophoresis. Each of the 20 methicillin-susceptible strains had a unique SmaI pattern, but the 27 MRSA strains showed only seven SmaI patterns. More than half of the SmaI fragments in all of these seven patterns were identical, as were those in the patterns from two unrelated MRSA strains. Digestion with ApaI, which cuts staphylococcus DNA into at least twice as many fragments, confirmed the results obtained with SmaI. Lastly, the plasmid contents of MRSA strains showing identical SmaI and ApaI electrophoretic patterns were not identical. These results are interpreted as supporting the hypothesis that all MRSA strains arose from a single clone and emphasize the need to use several methods in epidemiological investigations of MRSA outbreaks.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Staphylococcus aureus
/
DNA Bacteriano
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
Limite:
Humans
País/Região como assunto:
Europa
Idioma:
En
Revista:
J Clin Microbiol
Ano de publicação:
1992
Tipo de documento:
Article
País de afiliação:
França