Batch cultures of recombinant Lactococcus lactis subsp. lactis in a stirred fermentor. II. Plasmid transfer in mixed cultures.
Appl Microbiol Biotechnol
; 37(3): 364-8, 1992 Jun.
Article
em En
| MEDLINE
| ID: mdl-1368910
ABSTRACT
The transfer of plasmids was studied in a stirred fermentor in the course of mixed batch cultures combining recombinant strains of Lactococcus lactis subsp. lactis (donor strains) with L. lactis subsp. lactis CNRZ 268M3 (recipient strain). Donor strains contained one or two of the following plasmids (coding for erythromycin or chloramphenicol resistance) pIL205 (self-transmissible), pIL252, pIL253 (non-transmissible but mobilizable by pIL205, respectively small and large copy number) and pE194 (inserted in the chromosome). Only self-transmissible plasmid pIL205 was transferred, with frequencies ranging from 10(-7) to 10(-8) after 12 h of fermentation. These frequencies were 60-400 times lower than in unstirred M17 broth and 100,000 times lower than on agar medium. In the latter case, non-transmissible plasmids pIL252 and pIL253 were mobilized by pIL205 with a frequency of about 10(-5) - 10(-6).
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Lactococcus lactis
Idioma:
En
Revista:
Appl Microbiol Biotechnol
Ano de publicação:
1992
Tipo de documento:
Article
País de afiliação:
França