The use of oligonucleotide probes containing 2'-deoxy-2'-fluoronucleosides for regiospecific cleavage of RNA by RNase H from Escherichia coli.
Biochim Biophys Acta
; 1130(1): 41-6, 1992 Feb 28.
Article
em En
| MEDLINE
| ID: mdl-1371935
ABSTRACT
Protected 2'-deoxy-2'-fluorouridine and 2'-deoxy-2'-fluorocytidine suitable for incorporation into oligonucleotides via the phosphoramidite approach have been prepared. Five modified and two unmodified oligonucleotides have been synthesized to investigate the regiospecific cleavage of a 5S RNA from Escherichia coli by RNase H. In order to show whether the modified oligonucleotides are able to hybridize with the RNA the physico-chemical properties (melting curves, CD spectra) of analogous DNA/oligodeoxyribonucleotide duplexes have been examined. The modified oligonucleotides are shown to form stable duplexes with a DNA-matrix which exist in an A-like form. Two of the modified probes containing four 2'-deoxy-2'-fluorocytidines or two 2'-deoxy-2'-fluorouridines direct the splitting by RNase H of only one phosphodiester bond of the RNA.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
RNA Bacteriano
/
Sondas de Oligonucleotídeos
/
Floxuridina
/
Ribonuclease H
/
Desoxicitidina
Idioma:
En
Revista:
Biochim Biophys Acta
Ano de publicação:
1992
Tipo de documento:
Article
País de afiliação:
Alemanha