Enhanced hematopoietic growth factor production in an experimental myeloproliferative syndrome.
Blood
; 79(12): 3179-87, 1992 Jun 15.
Article
em En
| MEDLINE
| ID: mdl-1375844
ABSTRACT
The murine myeloproliferative syndrome induced by the myeloproliferative sarcoma virus (MPSV) has numerous similarities to human primary myelofibrosis. We have shown that medium conditioned by spleen cells of MPSV-infected mice has the capacity to support the growth of primitive blast cell colonies. The detection of this activity associated with MPSV infection stimulated us to characterize the hematopoietins responsible for this activity. Northern blot analysis showed a large increase, or induction, of interleukin-6 (IL-6), granulocyte-macrophage colony-stimulating factor (GM-CSF), macrophage-CSF (CSF-1), and granulocyte-CSF (G-CSF) transcripts in the hematopoietic organs of MPSV-infected mice; however, no IL-3 transcript could be detected in either MPSV-infected or normal mice. Significant levels of IL-1 alpha, IL-6, G-CSF, and CSF-1 bioactivities were found in the serum of MPSV-infected mice, but not in controls. Additionally, analysis of medium conditioned by spleen cells of MPSV-infected mice showed the presence of tumor necrosis factor alpha bioactivity. The increased production of cytokines that are able to stimulate pluripotent hematopoietic stem cells corroborates the hypothesis of a possible involvement of hematopoietic growth factors in the development of some myeloproliferative disorders.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fatores de Crescimento de Células Hematopoéticas
/
Transtornos Mieloproliferativos
Limite:
Animals
Idioma:
En
Revista:
Blood
Ano de publicação:
1992
Tipo de documento:
Article
País de afiliação:
França