Membrane-derived oligosaccharides (MDO's) promote closing of an E. coli porin channel.
FEBS Lett
; 304(2-3): 216-20, 1992 Jun 15.
Article
em En
| MEDLINE
| ID: mdl-1377642
ABSTRACT
The outer membrane of Escherichia coli is a diffusion barrier for macromolecules, but allows the passage of small hydrophilic solutes through non-specific channels, the porins. Some electrophysiological studies find reconstituted porins in a mostly open state, while those done with the patch-clamp technique performed on live cells suggest that the vast majority of the native channels are closed. We present here current measurements through porins from reconstituted outer membrane, which demonstrate that bacterial metabolites, the MDO's, which bathe the periplasmic side of the outer membrane, induce the channels to close. These findings illustrate that the degree of openness of porins can be regulated by compounds naturally found in bacteria.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oligossacarídeos
/
Proteínas da Membrana Bacteriana Externa
/
Escherichia coli
/
Canais Iônicos
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
1992
Tipo de documento:
Article