Cellular localization of mRNA for cellular retinoic acid-binding protein II and nuclear retinoic acid receptor-gamma 1 in retinoic acid-treated human skin.
J Invest Dermatol
; 99(2): 146-50, 1992 Aug.
Article
em En
| MEDLINE
| ID: mdl-1378478
ABSTRACT
We have previously shown that cellular retinoic acid-binding protein II (CRABP-II), but not cellular retinoic acid-binding protein I (CRABP-I), mRNA expression is markedly induced in human skin by topical retinoic acid. In the present study, we have investigated the pattern of expression of CRABP-II transcripts in 4-d RA-treated human skin by non-radioactive in situ hybridization (n = 5) and Northern analysis (n = 4). RA induced accumulation of CRABP-II transcripts throughout the epidermis, dermal fibroblasts, and endothelial cells as determined by in situ hybridization. In skin treated with vehicle, a faint hybridization signal was observed only in basal layers of the epidermis consistent with low-level expression of CRABP-II mRNA. RA-mediated accumulation of CRABP-II transcripts in skin was also confirmed by Northern analysis. Neither RA nor vehicle induced significant changes in nuclear RA receptor-gamma 1 or keratin 5 gene expression in skin as determined by in situ or Northern hybridization. These results indicate that RA-induced CRABP-II mRNA accumulation is primarily localized to spinous and granular layers in epidermis, and in superficial dermis.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pele
/
Tretinoína
/
RNA Mensageiro
/
Proteínas de Transporte
Tipo de estudo:
Clinical_trials
Limite:
Humans
Idioma:
En
Revista:
J Invest Dermatol
Ano de publicação:
1992
Tipo de documento:
Article