Troponin T isoform expression in the normal and failing human left ventricle: a correlation with myofibrillar ATPase activity.
Basic Res Cardiol
; 87 Suppl 1: 117-27, 1992.
Article
em En
| MEDLINE
| ID: mdl-1386729
ABSTRACT
The expression of troponin T, a thin filament regulatory protein, was examined in normal and failing left ventricles. The samples were obtained from the hearts of patients with severe heart failure who were undergoing cardiac transplantation, and from normal adult hearts that could not be used for transplantation. Western blots of the myofibrillar proteins demonstrated two isoforms, troponin T 1 (TnT1) and troponin T 2 (TnT2). TnT2 is expressed at significantly higher levels in failing hearts (p less than 0.004). Western blots of two-dimension SDS-PAGE gels resolved two dominant spots of TnT1 and of TnT2 and several minor troponin T species. Alkaline phosphatase treatment markedly decreased the sizes of the two acidic spots while increasing the two more basic spots by a comparable amount. Myofibrillar ATPase activity had an inverse and negative linear relationship (r = 0.7, p less than 0.02) with the myofibrillar percentage of total troponin T comprised of TnT2. In that heart failure in these transplant patients had multiple bases, we propose that rather than a cause of heart failure, the disease-associated changes in troponin T isoform expression are an adaptation to abnormal myocardial function.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Troponina
/
Miosinas
/
Insuficiência Cardíaca
/
Ventrículos do Coração
Limite:
Adolescent
/
Adult
/
Female
/
Humans
/
Male
/
Middle aged
Idioma:
En
Revista:
Basic Res Cardiol
Ano de publicação:
1992
Tipo de documento:
Article