Molecular analysis of the 3' terminal region of the genome of Beet mosaic virus and its relation with other potyviruses.

ABSTRACT

A1633 nucleotides long 3'-terminal sequence of genome of the Beet mosaic virus (BtMV) Ren1 isolate from Slovakia, spanning a part of the NIb gene, complete capsid protein (CP) gene and a 3'non-coding region was determined. Computer-assisted translation resulted in a polypeptide encompassing typical conserved potyviral amino acid (aa) motifs. Homology BLAST search and phylogenetic analysis revealed that BtMV was closest to Bean common mosaic virus, Soybean mosaic virus and Zucchini yellow mosaic virus. An unexpectedly high, 99.6% aa sequence identity was found between BtMV-Ren1 and a previously sequenced British BtMV isolate. Restriction analysis and sequencing of the CP gene of other Slovak BtMV isolates revealed their high homology. Developed reverse transcription-polymerase chain reaction protocols enabled sensitive, rapid and reliable detection of the virus.