Imaging molecular interactions in cells by dynamic and static fluorescence anisotropy (rFLIM and emFRET).
Biochem Soc Trans
; 31(Pt 5): 1020-7, 2003 Oct.
Article
em En
| MEDLINE
| ID: mdl-14505472
ABSTRACT
We report the implementation and exploitation of fluorescence polarization measurements, in the form of anisotropy fluorescence lifetime imaging microscopy (rFLIM) and energy migration Förster resonance energy transfer (emFRET) modalities, for wide-field, confocal laser-scanning microscopy and flow cytometry of cells. These methods permit the assessment of rotational motion, association and proximity of cellular proteins in vivo. They are particularly applicable to probes generated by fusions of visible fluorescence proteins, as exemplified by studies of the erbB receptor tyrosine kinases involved in growth-factor-mediated signal transduction.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Anisotropia
/
Microscopia de Fluorescência
Tipo de estudo:
Prognostic_studies
/
Risk_factors_studies
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Biochem Soc Trans
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
Alemanha