Recombinant protein to analyze autoantibodies to proteinase 3 in systemic vasculitis.
Am J Clin Pathol
; 120(4): 586-95, 2003 Oct.
Article
em En
| MEDLINE
| ID: mdl-14560570
ABSTRACT
The presence of antineutrophil cytoplasmic autoantibodies with specificity for proteinase 3 (PR3-ANCA) usually is detected by enzyme-linked immunosorbent assay (ELISA) with purified PR3 as a substrate. We studied the technical performance of direct and capture ELISA using a recombinant proteolytically inactive form of PR3 produced in the baculovirus expression system for the detection of PR3-ANCA in 114 patients with systemic vasculitis at diagnosis. We found that ELISA using recombinant PR3 produced in insect cells is a promising alternative for ELISA with native PR3. We found a correlation between tests using recombinant or native PR3, as well as correlation of the ELISA results with ANCA titers measured by the indirect immunofluorescence technique. However, the specificity for ANCA-associated vasculitis of ELISA with recombinant PR3 was lower than ELISA using native PR3. Compared with the direct assay, capture ELISA is a more sensitive method for PR3-ANCA detection, with both native and recombinant PR3, and its results depend on the monoclonal antibody used to capture the antigen.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vasculite
/
Proteínas Recombinantes
/
Ensaio de Imunoadsorção Enzimática
/
Serina Endopeptidases
/
Anticorpos Anticitoplasma de Neutrófilos
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
/
Female
/
Humans
/
Male
Idioma:
En
Revista:
Am J Clin Pathol
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
Holanda