Identification of a type-D feruloyl esterase from Neurospora crassa.
Appl Microbiol Biotechnol
; 63(5): 567-70, 2004 Feb.
Article
em En
| MEDLINE
| ID: mdl-14595525
ABSTRACT
Feruloyl esterases constitute an interesting group of enzymes that have the potential for use over a broad range of applications in the agri-food industries. In order to expand the range of available enzymes, we have examined the presence of feruoyl esterase genes present in the genome sequence of the filamentous fungus Neurospora crassa. We have identified an orphan gene (contig 3.544), the translation of which shows sequence identity with known feruloyl esterases. This gene was cloned and the corresponding recombinant protein expressed in Pichia pastoris to confirm that the enzyme (NcFaeD-3.544) exhibits feruloyl esterase activity. Unusually the enzyme was capable of p-coumaric acid release from untreated crude plant cell wall materials. The substrate utilisation preferences of the recombinant enzyme place it in the recently recognised type-D sub-class of feruloyl esterase.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Hidrolases de Éster Carboxílico
/
Neurospora crassa
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Appl Microbiol Biotechnol
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
Reino Unido
País de publicação:
ALEMANHA
/
ALEMANIA
/
DE
/
DEUSTCHLAND
/
GERMANY