Effects of atmospheric ozone on microarray data quality.
Anal Chem
; 75(17): 4672-5, 2003 Sep 01.
Article
em En
| MEDLINE
| ID: mdl-14632079
ABSTRACT
A data anomaly was observed that affected the uniformity and reproducibility of fluorescent signal across DNA microarrays. Results from experimental sets designed to identify potential causes (from microarray production to array scanning) indicated that the anomaly was linked to a batch process; further work allowed us to localize the effect to the posthybridization array stringency washes. Ozone levels were monitored and highly correlated with the batch effect. Controlled exposures of microarrays to ozone confirmed this factor as the root cause, and we present data that show susceptibility of a class of cyanine dyes (e.g., Cy5, Alexa 647) to ozone levels as low as 5-10 ppb for periods as short as 10-30 s. Other cyanine dyes (e.g., Cy3, Alexa 555) were not significantly affected until higher ozone levels (> 100 ppb). To address this environmental effect, laboratory ozone levels should be kept below 2 ppb (e.g., with filters in HVAC) to achieve high quality microarray data.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ozônio
/
Atmosfera
/
Carbocianinas
/
Artefatos
/
Análise de Sequência com Séries de Oligonucleotídeos
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Anal Chem
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
Estados Unidos