[Study on exons 3 and 4 of alpha-synuclein gene in Chinese familial Parkinson disease patients].
Zhonghua Yi Xue Yi Chuan Xue Za Zhi
; 20(6): 536-8, 2003 Dec.
Article
em Zh
| MEDLINE
| ID: mdl-14669226
ABSTRACT
OBJECTIVE:
To study the relationship of Chinese familial Parkinson disease with alpha-synuclein gene.METHODS:
Polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) and polymerase chain reaction-heteroduplex analysis(PCR-HA) were employed to detect the abnormal mobilization in the familial Parkinson disease and sporadic Parkinson disease patients, then it was verified by gene sequencing.RESULTS:
No mutation was found in alpha-synuclein gene exons 3 and 4 by PCR-SSCP together with PCR-HA. An inserted c and an inserted t were found in intron 4, position 23 and position 67 respectively.CONCLUSION:
(1) Exons 3 and 4 of alpha-synuclein gene are not the mutational hot spots of Chinese familial Parkinson disease. (2) Two polymorphisms were found in intron 4 of alpha-synuclein gene. They are 23 ins c and 67 ins t.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Doença de Parkinson
/
Éxons
/
Proteínas do Tecido Nervoso
Limite:
Adult
/
Aged
/
Female
/
Humans
/
Male
/
Middle aged
Idioma:
Zh
Revista:
Zhonghua Yi Xue Yi Chuan Xue Za Zhi
Assunto da revista:
GENETICA MEDICA
Ano de publicação:
2003
Tipo de documento:
Article