[Epidemiology of Chlamydia trachomatis using analysis of gene encoding of the major outer membrane protein]. / Epidémiologie de Chlamydia trachomatis par l'analyse du gène de la protéine majeure de membrane externe.
Pathol Biol (Paris)
; 40(5): 583-9, 1992 May.
Article
em Fr
| MEDLINE
| ID: mdl-1495847
ABSTRACT
One hundred and eight clinical strains and 24 reference strains of C. trachomatis were typed using differential restriction mapping of omp1, the gene which encodes the major outer membrane protein. The gene was obtained by polymerase chain reaction (PCR). This molecular typing method correlated well with serological typing. Eighty-four per cent of clinical strains were typed using the enzyme AluI alone. Heterogeneity was looked for among the most common serovars (E, F, and D; 62%, 17%, and 9%, respectively). Analysis of the PCR-amplified fourth variable domain of omp1 using denaturing gradient gel electrophoresis followed by direct sequencing of the variants disclosed substantial heterogeneity within the D serovar. Conversely, serovars E and F were homogeneous, with however a single variant strain of serovar E.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas da Membrana Bacteriana Externa
/
DNA Bacteriano
/
Chlamydia trachomatis
Tipo de estudo:
Screening_studies
Idioma:
Fr
Revista:
Pathol Biol (Paris)
Ano de publicação:
1992
Tipo de documento:
Article