Adenosine triphosphate concentration and beta-D-glucuronidase activity as indicators of sea bass semen quality.
Biol Reprod
; 70(6): 1679-84, 2004 Jun.
Article
em En
| MEDLINE
| ID: mdl-14960478
ABSTRACT
The most common parameters used to evaluate sperm quality are motility rate and duration and fertilization ability. In this study, chemical and biochemical parameters of sea bass (Dicentrarchus labrax) sperm were investigated to find an alternative method for evaluating sperm fertilization ability before and after cryopreservation. The biochemical and chemical analyses were performed with fresh and frozen-thawed sperm and seminal plasma. To cryopreserve sperm, 250-microl straws were used. Fertilization ability was evaluated by inseminating eggs (obtained from hormonally stimulated females) with fresh and cryopreserved sperm. The results revealed a linear relationship (P < 0.05) between semen fertilization capacity and some seminal plasma (beta-D-glucuronidase activity, potassium concentration) and sperm (ATP concentration, aspartate aminotransferase activity) parameters. Variations in semen fertilization rate could be best described by two multiple regression models one including the sperm parameters and another including the seminal plasma parameters. For practical application, the use of simple regression models is of value. Fertilization rate in both fresh and cryopreserved sperm was reliably predicted by determining the ATP concentration or the beta-D-glucuronidase activity or both.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sêmen
/
Bass
/
Trifosfato de Adenosina
/
Glucuronidase
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Biol Reprod
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
Itália