The Q-Trap mass spectrometer, a novel tool in the study of protein glycosylation.
J Am Soc Mass Spectrom
; 15(3): 413-23, 2004 Mar.
Article
em En
| MEDLINE
| ID: mdl-14998545
ABSTRACT
The use of the recently introduced Q-Trap mass spectrometer in the study of protein glycosylation is described. The combined ion trap and triple quadrupole scan functions make it a powerful system in both oligosaccharide and glycopeptide analysis. Several oligosaccharides, both linear and branched, were analyzed to obtain information on sequence, linkage, and branching. Quadrupole like MS/MS spectra with ion trap sensitivity but without the typical ion trap low mass cut-off were obtained. To determine the origin of fragments and to reveal the existence of new ions, the MS(3) capabilities of the system proved to be useful. Glycopeptides were selectively detected in peptide mixtures using the triple quadrupole precursor ion scan function, either in off-line experiments or during LC/MS using information dependent acquisition (IDA).
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polissacarídeos
/
Espectrometria de Massas
/
Proteínas
Limite:
Animals
Idioma:
En
Revista:
J Am Soc Mass Spectrom
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
Bélgica