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Bioreactor culture of oil palm (Elaeis guineensis) and effects of nitrogen source, inoculum size, and conditioned medium on biomass production.
Gorret, Nathalie; bin Rosli, Samsul Kamal; Oppenheim, Sheldon F; Willis, Laura B; Lessard, Philip A; Rha, ChoKyun; Sinskey, Anthony J.
Afiliação
  • Gorret N; Department of Biology, Massachusetts Institute of Technology, Room 68-370, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.
J Biotechnol ; 108(3): 253-63, 2004 Mar 18.
Article em En | MEDLINE | ID: mdl-15006426
ABSTRACT
We report the successful culture of oil palm (Elaeis guineensis Jacq.) suspension cells in a bioreactor. In vitro propagation of this perennial monocotyledonous tree is an important part of the oil palm industry's approach to clonal propagation of high-yielding accessions. During culture of oil palm cells in a batch bioreactor, nutrients and extracellular metabolites were monitored, and kinetic parameters and nutrient-to-biomass conversion yields were calculated. The biomass increased approximately 3.5-fold per month, consistent with values reported for shake flask cultures. Although the carbon source was completely depleted by the end of the run, nitrogen sources remained in large excess and the sugar-to-biomass conversion yield remained low. Linear growth indicated that the cells were limited. The results obtained from the bioreactor runs indicated that we should be able to improve biomass production by carrying out optimization studies. Therefore, we initiated multi-factorial analyses using response surface experimental designs to investigate the effects of different nitrogen sources, as well as inoculum size and conditioned medium, on biomass production in flask cultures. Whereas glutamine does not have a significant effect on biomass production, ammonia has a positive effect up to an optimum concentration. Both inoculum density and conditioned medium have positive, synergistic effects on biomass production.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Técnicas de Cultura de Células / Reatores Biológicos / Arecaceae / Nitrogênio Tipo de estudo: Evaluation_studies / Prognostic_studies Idioma: En Revista: J Biotechnol Assunto da revista: BIOTECNOLOGIA Ano de publicação: 2004 Tipo de documento: Article País de afiliação: Estados Unidos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Técnicas de Cultura de Células / Reatores Biológicos / Arecaceae / Nitrogênio Tipo de estudo: Evaluation_studies / Prognostic_studies Idioma: En Revista: J Biotechnol Assunto da revista: BIOTECNOLOGIA Ano de publicação: 2004 Tipo de documento: Article País de afiliação: Estados Unidos