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UVA activated 8-MOP and chlorpromazine inhibit release of TNF-alpha by post-transcriptional regulation.
Wolnicka-Glubisz, Agnieszka; Sarna, Tadeusz; Klosner, Gabriele; Knobler, Robert; Trautinger, Franz.
Afiliação
  • Wolnicka-Glubisz A; Department of Biophysics, Faculty of Biotechnology, Jagiellonian University, Krakow, Poland.
Photochem Photobiol Sci ; 3(4): 334-6, 2004 Apr.
Article em En | MEDLINE | ID: mdl-15052360
There is evidence that regulation of inflammatory cytokines is among the immunomodulatory effects of photochemotherapy with 8-MOP and UVA. We have recently demonstrated that in the monocytoid cell line U937 incubation with 8-MOP and subsequent exposure to UVA is able to efficiently downregulate the release of TNF-alpha into the culture supernatant. Chlorpromazine, a well known photosensitising drug, was even more potent with regard to this effect. Based on these observations, in this study we further investigate the mechanisms of TNF-alpha inhibition by 8-MOP and CPZ photosensitization. For this purpose we determined intracellular protein levels and gene expression of TNF-alpha by western blot and quantitative real-time PCR, respectively. Our results indicate that the observed inhibition of TNF-alpha secretion after photochemotherapy is not due to downregulation of gene transcription but rather to a post-transcriptional mechanism. The observed decrease of intracellular TNF-alpha with CPZ and 8-MOP points to decreased protein synthesis or enhanced degradation. These findings demonstrate that posttranscriptional regulation of cytokine expression is a possible mechanism of action of photochemotherapy.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fotoquimioterapia / Clorpromazina / Fator de Necrose Tumoral alfa / Metoxaleno Limite: Humans Idioma: En Revista: Photochem Photobiol Sci Assunto da revista: BIOLOGIA / QUIMICA Ano de publicação: 2004 Tipo de documento: Article País de afiliação: Polônia País de publicação: Reino Unido
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fotoquimioterapia / Clorpromazina / Fator de Necrose Tumoral alfa / Metoxaleno Limite: Humans Idioma: En Revista: Photochem Photobiol Sci Assunto da revista: BIOLOGIA / QUIMICA Ano de publicação: 2004 Tipo de documento: Article País de afiliação: Polônia País de publicação: Reino Unido