[Recombinant interleukin-3 expression system in E. coli]. / Sistemy ékspressii v E. coli rekombinantnogo interleikina-3.
Bioorg Khim
; 18(3): 391-7, 1992 Mar.
Article
em Ru
| MEDLINE
| ID: mdl-1524590
ABSTRACT
Plasmid pTOTE2IL3 (III) has been constructed, expressing an artificial human interleukin-3 (hIL3) gene under conditions of the induced protein biosynthesis. Levels of the recombinant protein synthesis have been compared in several E. coli strains containing expression plasmids pTE2IL3 (I) (constitutive biosynthesis) and (III) (induced biosynthesis). Optimal combinations of the expression plasmids and the bacterial strains are of importance. A simple and effective method has been elaborated for isolation, purification and renaturation of the recombinant protein accumulated in inclusion bodies.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Interleucina-3
/
Escherichia coli
Limite:
Humans
Idioma:
Ru
Revista:
Bioorg Khim
Ano de publicação:
1992
Tipo de documento:
Article