Phospholipase C activator m-3M3FBS affects Ca2+ homeostasis independently of phospholipase C activation.
Br J Pharmacol
; 143(1): 3-7, 2004 Sep.
Article
em En
| MEDLINE
| ID: mdl-15302681
ABSTRACT
In this study, we have investigated responses to the phospholipase C (PLC) activator m-3M3FBS in SH-SY5Y human neuroblastoma cells. As measured using fura-2, m-3M3FBS caused a slowly developing - full response was obtained within 4-6 min - Ca(2+) elevation both in the presence and absence of extracellular Ca(2+), indicating Ca(2+) release from intracellular stores, putatively from endoplasmic reticulum and mitochondria. PLC activity was also measured using two methods, the classical ion-exchange separation and the more novel fluorescent real-time method. In the time frame in which m-3M3FBS caused Ca(2+) elevation (up to 7 min), no PLC activation was detected. Instead, more than 20 min were required to see any inositol phosphate generation in response to m-3M3FBS. m-3M3FBS also interfered with store-operated Ca(2+) influx and Ca(2+) extrusion. In conclusion, m-3M3FBS cannot be considered either potent or specific PLC activator.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fosfolipases Tipo C
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Sulfonamidas
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Cálcio
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Ativadores de Enzimas
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Homeostase
Limite:
Animals
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Female
/
Humans
Idioma:
En
Revista:
Br J Pharmacol
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
Suécia