[Replication and encapsidation of HBV mutants with the truncated C gene].
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
; 18(1): 39-42, 2004 Mar.
Article
em Zh
| MEDLINE
| ID: mdl-15340524
ABSTRACT
OBJECTIVE:
To evaluate the replication and encapsidation of HBV mutants with the truncated C gene.METHODS:
The HBV mutants with the truncated C gene were constructed by molecular cloning and PCR-based deletion in vitro. The replication and encapsidation of HBV mutants were investigated by Southern blotting, PCR and real-time fluorescence PCR respectively after transfecting the HBV mutants plasmid into HepG2 cells by using liposome.RESULTS:
The C-truncated HBV mutant vectors were constructed successfully and confirmed exactly by clone sequencing and enzymes digestion. The C-truncated HBV mutants were replication defective, however, all types of HBV DNA could be detected positive in the cytoplasm and supernatant after co-transfecting the C-truncated HBV mutants plasmid and the helper constructs into HepG2 cells. The C-truncated HBV mutants were proved to produce 3-40 folds more progeny DNA than that of the wild-type HBV by DNA quantitative assay.CONCLUSION:
The C-truncated HBV mutants are replication-deficient and could not replicate and encapsulate in the hepatocytes when transfected solely, however, the progeny HBV-variant viruses are encapsidated more effectively to secrete into supernatant when co-transfected with the helper construct which lacks part of 5 prime-proximal HBV RNA packaging signal Epsilon.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Replicação Viral
/
Vírus da Hepatite B
/
Antígenos do Núcleo do Vírus da Hepatite B
/
Mutação
Limite:
Humans
Idioma:
Zh
Revista:
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
Assunto da revista:
VIROLOGIA
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
China